人肝永生化细胞THLE-2

细胞名称

人肝永生化细胞THLE-2

货物编码

BFN60808733

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研     2类

培养体系

DMEM高糖+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人肝永生化细胞THLE-2取自男性供体，经过SV40永生化处理。该细胞源于ATCC

注释

倍增时间33h

基因突变

/

HLA信息

STR信息

Amelogenin        X,Y

CSF1PO        11,13

D5S818        11,13

D7S820        10,12

D13S317        8,12

D16S539        11,13

TH01        7,9.3

TPOX        8,11

vWA        16,17

参考文献

PubMed=7685115; DOI=10.1073/pnas.90.11.5123

Pfeifer A.M.A., Cole K.E., Smoot D.T., Weston A., Groopman J.D., Shields P.G., Vignaud J.M., Juillerat M., Lipsky M.M., Trump B.F., Lechner J.F., Harris C.C.

Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.

Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833

Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.

p53 mutations in human immortalized epithelial cell lines.

Carcinogenesis 14:833-839(1993)

Patent=US5759765

Harris C.C., Cole K.H., Lechner J.F., Reddel R.R.

Human liver epithelial cell lines.

Patent number US5759765, 02-Jun-1998

PubMed=23887712; DOI=10.1038/ncomms3218

Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.

High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.

Nat. Commun. 4:2218-2218(2013)