人急性早幼粒白血病细胞NB4

细胞名称

人急性早幼粒白血病细胞NB4

货物编码

BFN60805251

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研           1类

培养体系

DMEM高糖+10%FBS+1%双抗

培养温度

37℃

二氧化碳浓度

5%

简介

人急性早幼粒白血病细胞NB4取自23岁女性供体，引种自DSMZ

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 3.

Part of: LL-100 blood cancer cell line panel.

Registration: International Depositary Authority, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ); ACC-2030.

Registration: International Depositary Authority, Pasteur Institute Collection Nationale de Cultures de Micro-organismes (CNCM); I-1045.

Doubling time: 36-40 hours (PubMed=1995093); 30 hours (PubMed=25984343); ~35-45 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Cell surface proteome.

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Bone marrow.

STR信息

Amelogenin        X

CSF1PO        11,12

D3S1358        15,17

D5S818        13

D7S820        10,13

D8S1179        10,14

D13S317        11,12

D16S539        9

D18S51        12,14

D21S11        28,33.2

FGA        21,22

Penta D        10,13

Penta E        7,13

TH01        7,9.3

TPOX        8,11

vWA        16,19

参考文献

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x

Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.

The LL-100 panel: 100 cell lines for blood cancer studies.

Sci. Rep. 9:8218-8218(2019)