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简要描述:青旗(上海)生物技术发展有限公司,总部位于上海浦东新区,依托本地高校资源,逐步发展成为以生物技术为主的研发、生产、培训为一体的综合化产业平台,在标准化细胞库建立及细胞药物前端模型方面成果显著。公司生产经营原代细胞、细胞系、ELISA试剂盒、感受态细胞和HPLC检测等科研产品与服务。我们秉承对用户负责的态度,以对科研的高度严谨,以严格的质量控制,为广大生物医学科研用户提供更优质的服务!
更新时间:2021-05-26
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品牌 | 其他品牌 | 货号 | BFN607200285 |
---|---|---|---|
规格 | T25培养瓶x1 1.5ml冻存管x2 | 供货周期 | 现货 |
主要用途 | 仅供科研 | 应用领域 | 医疗卫生,生物产业 |
细胞名称 | 人脐静脉内皮细胞 HUVEC | ||
货物编码 | BFN607200285 | ||
产品规格 | T25培养瓶x1 | 1.5ml冻存管x2 | |
细胞数量 | 1x10^6 | 1x10^6 | |
保存温度 | 37℃ | -198℃ | |
运输方式 | 常温保温运输 | 干冰运输 | |
安全等级 | 1 | ||
用途限制 | 仅供科研用途 1类 |
培养体系 | The base medium for this cell line is F-12K Medium (ATCC 30-2004). To make the complete growth medium, add the following components to 440 mL of the base medium:
5 mL of a 10 mg/mL stock heparin solution (prepared from Sigma catalog #H3393) for a final concentration of 0.1 mg/mL heparin in complete growth medium Dissolve 1 g Heparin in 100 mL basal F-12K and filter to make a 10 mg/mL stock solution 5 mL endothelial cell growth supplement (ECGS; BD Biosciences catalog # 354006) Rehydrate 1 vial using 5 mL basal F-12K 50 mL fetal bovine serum (FBS; ATCC 30-2020) | ||
培养温度 | 37℃ | 二氧化碳浓度 | 5% |
简介 | 人脐静脉内皮细胞HUVEC 源于ATCC(CRL-1730).部分文献称该细胞为HUVEC-C。 | ||
注释 | Characteristics: Life expectancy of 50 to 60 population doublings (ATCC). 54 PDL (PubMed=28755030). Characteristics: Contains an integrated copy of HHV-6B (CI-HHV-6B) at the distal end of chromosome 9 (PubMed=28755030). Doubling time: ~23.5 hours (between passages 18 and 30), ~67 hours (between passages 24 and 27), ~84 hours (between passages 27 and 30), ~100 hours (between passages 32 and 34) (PubMed=28755030). | ||
STR信息 | / | ||
参考文献 | PubMed=6333682; DOI=10.1073/pnas.81.20.6413 Hoshi H., McKeehan W.L. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. U.S.A. 81:6413-6417(1984)
PubMed=28755030; DOI=10.1007/s10616-017-0119-y Shioda S., Kasai F., Ozawa M., Hirayama N., Satoh M., Kameoka Y., Watanabe K., Shimizu N., Tang H., Mori Y., Kohara A. The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture. Cytotechnology 70:141-152(2018)
PubMed=29892436; DOI=10.1098/rsos.172472 Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A. Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection. R. Soc. Open Sci. 5:172472-172472(2018) |
青旗(上海)生物技术发展有限公司,总部位于上海浦东新区,依托本地高校资源,逐步发展成为以生物技术为主的研发、生产、培训为一体的综合化产业平台,在标准化细胞库建立及细胞药物前端模型方面成果显著。公司生产经营原代细胞、细胞系、ELISA试剂盒、感受态细胞和HPLC检测等科研产品与服务。我们秉承对用户负责的态度,以对科研的高度严谨,以严格的质量控制,为广大生物医学科研用户提供更优质的服务!